Glade Creek is an urban-type, coldwater stream located in the Blue Ridge geologic province of Virginia, within the counties of Roanoke and Botetourt with portions flowing through the City of Roanoke and the Town of Vinton. The Glade Creek Watershed encompasses over 21,000 acres and Glade Creek proper flows for about 12.5 miles starting in Botetourt County and emptying into Tinker Creek about 1 mile upstream from where Tinker Creek enters the Roanoke River.
Over 150 Roanoke Valley residents live right along the banks of Glade Creek and call it home, while over 20,000 Roanoke Valley residents live within the watershed. The stream is a popular fishery for trout, large and small mouth bass, and bluegill. It is home to numerous species of fish, including the Federally Endangered Roanoke Logperch (Percina rex). The Riparian area along its streambanks provide valuable habitat for wildlife including: birds, amphibians (frogs and salamanders), and mammals, including rabbits, squirrels and deer.